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Part:BBa_K863206:Design

Designed by: Julia Schirmacher   Group: iGEM12_Bielefeld-Germany   (2012-09-23)

Mating factor alpha 1 with Kozak sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 256
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers for isolation of the sequence with BioBrick Prefix in the fwd primer and Suffix in the rev primer.
fwd: 5'-ACGTgaattcgcggccgcttctagagcccgccgccaccATGAGATTTCCTTCAATTTT-3'
rev: 5'-ACGTCTGCAGCGGCCGCTACTAGTAAGCTTCAGCCTCTCTTTTCT-3'

Source

The Kozak sequence is based on the BioBrick BBa_J63003, but the sequence is used without the last bases atggag. The MFalpha1 sequence is from the plasmid pPICZalpha A from Invitrogen.

References